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(A) A xenic media; adult rrf3 worms were fed bacteria expressing control L4440 or cbp-1 dsRNA for 5 d, then transferred to liquid axenic media. (B) eat-2 mutation; adult eat-2 mutant worms (ad1113) were fed bacteria expressing control L4440 or cbp-1 dsRNA. (C) Bacterial dilution (bDR); adult worms were grown in liquid media at three concentrations of bacteria expressing control L4440 or cbp-1 dsRNA. (D) daf-2 mutant or wild-type worms under standard conditions were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi only partly blocks lifespan extension by the daf-2 mutation; p <0.05). (E) daf-2 mutant worms under optimum bacterial concentration (bDR) were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi blocks lifespan extension by the both bDR and the daf-2 mutation in these conditions). (F) Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant). (G) cbp-1 or control RNAi in <t>daf-16</t> hypomorphic worms (effect not significant).
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(A) A xenic media; adult rrf3 worms were fed bacteria expressing control L4440 or cbp-1 dsRNA for 5 d, then transferred to liquid axenic media. (B) eat-2 mutation; adult eat-2 mutant worms (ad1113) were fed bacteria expressing control L4440 or cbp-1 dsRNA. (C) Bacterial dilution (bDR); adult worms were grown in liquid media at three concentrations of bacteria expressing control L4440 or cbp-1 dsRNA. (D) daf-2 mutant or wild-type worms under standard conditions were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi only partly blocks lifespan extension by the daf-2 mutation; p <0.05). (E) daf-2 mutant worms under optimum bacterial concentration (bDR) were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi blocks lifespan extension by the both bDR and the daf-2 mutation in these conditions). (F) Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant). (G) cbp-1 or control RNAi in daf-16 hypomorphic worms (effect not significant).

Journal: PLoS Biology

Article Title: Role of CBP and SATB-1 in Aging, Dietary Restriction, and Insulin-Like Signaling

doi: 10.1371/journal.pbio.1000245

Figure Lengend Snippet: (A) A xenic media; adult rrf3 worms were fed bacteria expressing control L4440 or cbp-1 dsRNA for 5 d, then transferred to liquid axenic media. (B) eat-2 mutation; adult eat-2 mutant worms (ad1113) were fed bacteria expressing control L4440 or cbp-1 dsRNA. (C) Bacterial dilution (bDR); adult worms were grown in liquid media at three concentrations of bacteria expressing control L4440 or cbp-1 dsRNA. (D) daf-2 mutant or wild-type worms under standard conditions were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi only partly blocks lifespan extension by the daf-2 mutation; p <0.05). (E) daf-2 mutant worms under optimum bacterial concentration (bDR) were fed bacteria expressing control L4440 or cbp-1 dsRNA ( cbp-1 RNAi blocks lifespan extension by the both bDR and the daf-2 mutation in these conditions). (F) Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant). (G) cbp-1 or control RNAi in daf-16 hypomorphic worms (effect not significant).

Article Snippet: Antibodies CBP (C-1): Sc-7300 and DAF-16 (ce-300): Sc-33738 were purchased from Santa Cruz Biotechnology Inc. Anti-Histone H4 (#07-108) and anti-H4 (Lys5) (07-327) were purchased from Upstate.

Techniques: Expressing, Mutagenesis, Concentration Assay

(A) daf-16 , hsf-1 , and dve-1 RNAi attenuate lifespan extension by bDR. (B) daf-16 and hsf-1 but not dve-1 RNAi blocks lifespan extension by the daf-2 mutation. (C) daf-16 , hsf-1 , and dve-1 RNAi decreases resistance to 50 mM paraquat. bDR induces expression of (D) daf-16 and (E) hsf-1 , but cbp-1 RNAi does not block this induction. However, cbp-1 RNAi blocks induction of (F), sod-3 , (G) sip-1 , and (I) W03F9.4/CPT-1 and inhibition of (H) Y71H10A.1 by bDR. Data are presented as mean ± SEM ( n = 4–6/group, * p <0.05).

Journal: PLoS Biology

Article Title: Role of CBP and SATB-1 in Aging, Dietary Restriction, and Insulin-Like Signaling

doi: 10.1371/journal.pbio.1000245

Figure Lengend Snippet: (A) daf-16 , hsf-1 , and dve-1 RNAi attenuate lifespan extension by bDR. (B) daf-16 and hsf-1 but not dve-1 RNAi blocks lifespan extension by the daf-2 mutation. (C) daf-16 , hsf-1 , and dve-1 RNAi decreases resistance to 50 mM paraquat. bDR induces expression of (D) daf-16 and (E) hsf-1 , but cbp-1 RNAi does not block this induction. However, cbp-1 RNAi blocks induction of (F), sod-3 , (G) sip-1 , and (I) W03F9.4/CPT-1 and inhibition of (H) Y71H10A.1 by bDR. Data are presented as mean ± SEM ( n = 4–6/group, * p <0.05).

Article Snippet: Antibodies CBP (C-1): Sc-7300 and DAF-16 (ce-300): Sc-33738 were purchased from Santa Cruz Biotechnology Inc. Anti-Histone H4 (#07-108) and anti-H4 (Lys5) (07-327) were purchased from Upstate.

Techniques: Mutagenesis, Expressing, Blocking Assay, Inhibition